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For the four-step gradient, layer 1 ml of each of 4%, 5.5%, 7% and 10% (w/v) iodixanol and layer the cell suspension on top OR for the two step gradient layer an equal volume of 9.6% (w/v) iodixanol on top of the cell suspension in 21.6% (w/v) iodixanol (see Notes 4 and 5)

Die topische Anwendung soll genau jene Signale wieder verstrken, die in jungen Jahren reichlich vorhanden waren ohne dabei in die Hormonregulation einzugreifen

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